Synergistic disinfectant compositions having enhanced antimicrobial efficacy and stability, and methods of using the same

ABSTRACT

A synergistic disinfectant composition comprises a C1-8 organic acid, an amino acid based surfactant, an anionic surfactant, and a stabilizing agent. The C1-8 organic acid may include two or more types of the C1-8 organic acids with at least one of the C1-8 organic acids is alpha hydroxyl acid. The disinfectant composition has an antimicrobial activity of log reduction of at least 2 under Biocidal Product Registration (BPR) standard EN13727, EN1276, EN13624 or EN1499, and/or the EPA standard that applies the “Quantitative Methods for Evaluating the Activity of Microbicides used on Hard, Non-Porous Surface” issued by the Organisation for Economic Co-operation and Development (OECD). In addition, the disinfectant composition may be stable during storage and retain their antimicrobial activity after at least one month at 40° C. When desired, the synergistic disinfectant composition may further comprise an oxidizing agent.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application is a divisional patent application of U.S. patentapplication Ser. No. 17/714,178, filed on Apr. 6, 2022, which is acontinuation patent application of U.S. patent application Ser. No.16/762,611 (now U.S. patent Ser. No. 11/330,819 B2) that is a NationalPhase of PCT International Application No. PCT/US2019/066283, filed onDec. 13, 2019 and claims priority to U.S. Provisional Application No.62/786,176 filed on Dec. 28, 2018, and U.S. Provisional Application No.62/923,650 filed Oct. 21, 2019; the content of these patent applicationsare incorporated by reference herein.

TECHNICAL FIELD

This disclosure relates to disinfectant compositions useful ininstitutional and healthcare applications.

BACKGROUND

Conventional antimicrobial compositions typically used in liquiddisinfectant often present limitations in reaching antimicrobialefficacies at the permitted levels. In addition, many of thecompositions pose formulation stability challenges, especially when theconcentrations of active ingredients are increased in order to achievehigher antimicrobial efficacy. This may result in unsatisfactory shelflife of the disinfectant compositions, as well as potential hazardsunder regular storage and use conditions. Some conventional liquiddisinfectants purportedly contain both antimicrobial efficacy andbiodegradability by using plant origin raw materials. These products,however, do not meet the expectations for desired product attributes(such as foaming profile) observed in commercial non-green, syntheticcompositions. Further, biodegradable and plant origin raw materialsoften produce negative olfactory aspects (such as unpleasant odor) inconventional disinfectant products, causing unsatisfactory userexperience and reducing the actual use of these products.

Therefore, there is a need for liquid disinfectant compositions thathave one or more of enhanced antimicrobial efficacy, longer shelf life,improved stability, biodegradability, satisfactory foaming profile,improved olfactory aspects, and safer for consumer use.

SUMMARY

In one aspect, a synergistic disinfectant composition is provided thatmay comprise about 0.1% to about 25.0% by weight of a C1-8 organic acid,about 0.1% to about 20.0% by weight of an amino acid based surfactant,about 0.1% to about 20.0% by weight of an anionic surfactant, about0.05% to about 20.0% by weight of a stabilizing agent, all based ontotal weight of the composition. Optionally, the disinfectantcomposition may comprise about 0.01% to about 5.0% by weight of anoxidizing agent based on total weight of the composition. In certainembodiments, the C1-8 organic acid comprises two or more types of C1-8organic acids. In further embodiments, the C1-8 organic acid comprisesan alpha hydroxyl acid.

In another aspect, a synergistic disinfectant composition is providedthat may comprise a C1-8 organic acid; an amino acid based surfactant; astabilizing agent; and an anionic surfactant, wherein the disinfectantcomposition has an antimicrobial activity of log reduction of at least 2under European standards to chemical disinfectants and antisepticsEN13727, EN1276 or EN1499 or other similar methodologies recognizedunder the biocidal product regulation (BPR regulation (EN)528 (2010),and/or the United States Environmental Protection Agency (EPA) standardthat applies the “Quantitative Methods for Evaluating the Activity ofMicrobicides used on Hard, Non-Porous Surface” issued by theOrganisation for Economic Co-operation and Development (OECD).Optionally, the disinfectant composition may further comprise anoxidizing agent. Furthermore, the disinfectant composition hassubstantially the same antimicrobial activity after at least one monthat 40° C. storage.

In yet another aspect, a method of disinfecting a surface is provided.The method may comprise applying a synergistic disinfectant compositionas disclosed herein to the surface.

Other aspects of the disclosure will become apparent by consideration ofthe detailed description.

DETAILED DESCRIPTION

The present disclosure generally relates to a synergistic disinfectantcomposition, in which the antimicrobial efficacy may be enhanced throughthe synergy between C1-8 organic acids and amino-acid based surfactants.The disclosed synergistic compositions may include a stabilizing agentto achieve the stability with desired shelf life and to maintain theantimicrobial activity for at least one month after being stored at 40°C. In addition, the antimicrobial efficacy of the disclosed disinfectantcomposition may further be enhanced through the synergistic effectbetween the organic acid and the stabilizing agent.

The terms “comprise(s),” “comprising,” “include(s),” “including,”“having,” “has,” “contain(s),” “containing,” and variants thereof, asused herein, are open-ended transitional phrases, terms, or words thatare meant to encompass the items listed thereafter and equivalentsthereof as well as additional items. The singular forms “a”, “and”, and“the” include plural references unless the context clearly dictatesotherwise. Where the term “comprising” is used, the present disclosurealso contemplates other embodiments “comprising”, “consisting of”, or“consisting essentially of” elements presented herein, whetherexplicitly set forth or not.

Any numerical range recited herein includes all values from the lowervalue to the upper value. For example, if a concentration range isstated as 1% to 50%, it is intended that values such as 2% to 40%, 10%to 30%, or 1% to 3%, etc., are expressly enumerated in thisspecification. These are only examples of what is specifically intended,and all possible combinations of numerical values between and includingthe lowest value and the highest value enumerated are to be consideredto be expressly stated in this application.

The modifier “about” used in connection with a quantity is inclusive ofthe stated value and has the meaning dictated by the context (forexample, it includes at least the degree of error associated with themeasurement of the particular quantity). The modifier “about” shouldalso be considered as disclosing the range defined by the absolutevalues of the two endpoints. For example, the expression “from about 2to about 4” also discloses the range “from 2 to 4.” The term “about” mayrefer to plus or minus 10% of the indicated number. For example, “about10%” may indicate a range of 9% to 11%, and “about 1” may mean from0.9-1.1. Other meanings of “about” may be apparent from the context,such as rounding off, so, for example “about 1” may also mean from 0.5to 1.4.

In general, the amount of a component in a composition as disclosedherein is expressed “by weight” which refers to the percentage of thecomponent's weight in the total weight of the composition. Unlessindicated otherwise, all concentrations are expressed as weightpercentage concentrations.

The term “effective amount” refers to an amount effective that wouldachieve a desired effect or result. For example, an effective amount ofa disinfectant composition may refer to the amount of such compositionto achieve a level of antimicrobial activity, which can be measured witha standardized test known in the art. An effective amount of adisinfectant composition may be determined by known methods and may varyaccording to factors such as the microbial strains, test media,temperature, and other conditions.

The term “substantially the same antimicrobial activity” means that theantimicrobial activity is within at least 80%, preferably at least 90%,with respect to the reference antimicrobial activity.

The term “substantially free”, “free”, “substantially no”, or “no”refers to a disinfectant composition that does not contain a particularcompound, or to which a particular compound has not been added to thedisinfectant composition. Should the particular compound be presentthrough contamination, the amount of such particular compound shall beless than 0.5% by weight, preferably less than 0.1% by weight.

Definitions of specific functional groups and chemical terms aredescribed in more detail below. For purposes of this disclosure, thechemical elements are identified in accordance with the Periodic Tableof the Elements, CAS version, Handbook of Chemistry and Physics, 75^(th)Ed., inside cover, and specific functional groups are generally definedas described therein. Additionally, general principles of organicchemistry, as well as specific functional moieties and reactivity, aredescribed in Organic Chemistry, Thomas Sorrell, University ScienceBooks, Sausalito, 1999; Smith and March March's Advanced OrganicChemistry, 5th Edition, John Wiley & Sons, Inc., New York, 2001; Larock,Comprehensive Organic Transformations, VCH Publishers, Inc., New York,1989; Carruthers, Some Modern Methods of Organic Synthesis, 3^(rd)Edition, Cambridge University Press, Cambridge, 1987; the entirecontents of each of which are incorporated herein by reference.

The disclosed disinfectant composition may comprise a C1-8 organic acid,an amino acid based surfactant, an anionic surfactant, a stabilizingagent, and optionally an oxidizing agent.

C1-8 Organic Acid

The term “C1-8 organic acid” as used herein refers to a carboxylic acidshaving 1-8 carbon atoms and one or more carboxy (—COOH) groups. Theorganic acid may have 0, 1, 2, or 3 carbon-carbon double bonds. Theorganic acid may have a straight or branched carbon chain, a cycliccarbon ring, or an aromatic carbon ring. The organic acid may beunsubstituted or substituted with one or more substituent groups, eachof which independently selected from hydroxy (—OH), amino, cyano,halogen, thiol, oxo (═O), and thioxo (═S). Suitable C1-8 organic acidsmay include, but are not limited to, formic acid, acetic acid, propionicacid, butyric acid, isobutyric acid, valeric acid, isovaleric acid,caproic acid, heptanoic acid, benzoic acid, salicylic acid, caprylicacid, or any combination thereof. Furthermore, the “C1-8 organic acid”may include at least one alpha hydroxy acid containing 1-8 carbon atoms.

The term “alpha hydroxy acid” as used herein refers to a C1-8 organicacid, as defined herein, having a hydroxy group (—OH) attached to acarbon atom adjacent to a carboxy (—COOH) group in the molecularstructure. The alpha hydroxy acid may be natural or synthetic compound.Examples of suitable alpha hydroxy acids include, but are not limitedto, lactic acid, formic acid, glycolic acid, citric acid, malic acid,mandelic acid, tartaric acid, or any combination thereof.

The disinfectant compositions disclosed herein may comprise about 0.1%to about 50.0% by weight of a C1-8 organic acid. The compositions maycomprise at least 0.1%, at least 0.3%, at least 0.5%, at least 1.0%, atleast 2.0%, at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%,at least 7.0%, at least 8.0%, at least 9.0%, at least 10.0%, at least12.0%, at least 15.0%, at least 20.0%, at least 30.0%, or at least 40.0%by weight of a C1-8 organic acid; and/or the compositions may compriseless than 50.0%, less than 40.0%, less than 30.0%, less than 25.0%, lessthan 20.0%, less than 15.0%, less than 12.0%, less than 10.0%, less than9.0%, less than 8.0%, less than 7.0%, less than 6.0%, less than 5.0%,less than 4.0%, less than 3.0%, less than 2.0%, or less than 1.0% byweight of a C1-8 organic acid. For example, the compositions maycomprise about 0.1% to about 50.0%, about 0.1% to about 40.0%, about0.1% to about 30.0%, about 0.1% to about 25.0%, about 0.1% to about20.0%, about 0.1% to about 15.0%, about 0.1% to about 10.0%, or about0.1% to about 5.0% by weight of a C1-8 organic acid. The compositionsmay comprise about 0.5% to about 50.0%, about 0.5% to about 40.0%, about0.5% to about 30.0%, about 0.5% to about 25.0%, about 0.5% to about20.0%, about 0.5% to about 15.0%, about 0.5% to about 10.0%, about 0.5%to about 5.0% by weight of a C1-8 organic acid. The compositions maycomprise about 1.0% to about 50.0%, about 1.0% to about 40.0%, about1.0% to about 30.0%, about 1.0% to about 25.0%, about 1.0% to about20.0%, about 1.0% to about 15.0%, about 1.0% to about 12.0%, about 1.0%to about 10.0%, about 1.0% to about 8.0%, about 1.0% to about 6.0%, orabout 1.0% to about 5.0% by weight of a C1-8 organic acid. In someembodiments, the compositions comprise about 1.0% to about 25.0% byweight of a C1-8 organic acid. In particular embodiments, thecompositions comprise about 0.5% to about 15.0% by weight, or about 1.0%to about 12.0% by weight of a C1-8 organic acid.

In some embodiments, the organic acid may comprise an alpha hydroxyacid. The disinfectant compositions may comprise at least 0.05%, atleast 0.1%, at least 0.2%, at least 0.5%, at least 1.0%, at least 2.0%,at least 3.0%, at least 4.0%, at least 5.0%, at least 6.0%, at least7.0%, at least 8.0%, at least 9.0%, at least 10.0%, at least 11.0%, atleast 12.0%, at least 13.0%, at least 14.0%, or at least 15.0% by weightof an alpha hydroxy acid; and/or the compositions may comprise less than20.0%, less than 15.0%, less than 14.0%, less than 13.0%, less than12.0%, less than 11.0%, less than 10%, less than 9.0%, less than 8.0%,less than 7.0%, less than 6.0%, less than 5.0%, less than 4.0%, lessthan 3.0%, less than 2.0%, less than 1.0%, or less than 0.5% by weightof an alpha hydroxy acid. For example, the compositions may compriseabout 0.05% to about 20.0%, about 0.05% to about 15.0%, about 0.05% toabout 14.0%, about 0.05% to about 13.0%, about 0.05% to about 12.0%,about 0.05% to about 11.0%, about 0.05% to about 10.0%, about 0.05% toabout 9.0%, about 0.05% to about 8.0%, about 0.05% to about 7.0%, about0.05% to about 6.0%, about 0.05% to about 5.0%, about 0.05% to about4.0%, about 0.05% to about 3.0%, about 0.05% to about 2.0%, or about0.05% to about 1.0% by weight of an alpha hydroxy acid. The compositionsmay comprise about 0.1% to about 20%, about 0.1% to about 15.0%, about0.1% to about 14.0%, about 0.1% to about 13.0%, about 0.1% to about12.0%, about 0.1% to about 11.0%, about 0.1% to about 10.0%, about 0.1%to about 9.0%, about 0.1% to about 8.0%, about 0.1% to about 7.0%, about0.1% to about 6.0%, about 0.1% to about 5.0%, about 0.1% to about 4.0%,about 0.1% to about 3.0%, about 0.1% to about 2.0%, or about 0.1% toabout 1.0% by weight of an alpha hydroxy acid. The compositions maycomprise about 0.2% to about 20.0%, about 0.5% to about 20.0%, about0.5% to about 15.0%, about 0.5% to about 14.0%, about 0.5% to about13.0%, about 0.5% to about 12.0%, about 0.5% to about 11.0%, about 0.5%to about 10.0%, about 0.5% to about 9.0%, about 0.5% to about 8.0%,about 0.5% to about 7.0%, about 0.5% to about 6.0%, about 0.5% to about5.0%, about 0.5% to about 4.0%, about 0.5% to about 3.0%, about 0.5% toabout 2.0%, or about 0.5% to about 1.0% by weight of an alpha hydroxyacid. In some embodiments, the compositions comprise about 0.05% toabout 20.0% by weight, or about 0.05% to about 15.0% by weight of analpha hydroxy acid. In particular embodiments, the compositions compriseabout 0.05% to about 12.0% by weight of an alpha hydroxy acid.

In some embodiments, the disclosed disinfectant composition comprises asingle types of C1-8 organic acid. In some embodiments, the discloseddisinfectant composition comprises two or more types of C1-8 organicacids, such as a mixture of lactic acid and formic acid. In some furtherembodiments, the disclosed disinfectant composition comprises two ormore types of C1-8 organic acids wherein at least one of the C1-8organic acids is alpha hydroxyl acid.

In some embodiments, the C1-8 organic acid comprises or consists ofalpha hydroxyl acid. In some embodiments, the C1-8 organic acid is amixture of at least one alpha hydroxy acid and at least one organic acidthat does not contain an alpha hydroxyl group, such as formic acid oracetic acid. For example, the compositions as disclosed herein maycomprise about 0.1% to about 25.0% by weight of C1-8 organic acid, whichincludes about 0.05% to about 15.0% by weight of an alpha hydroxy acidand at least one organic acid that does not contain an alpha hydroxygroup.

Amino Acid Based Surfactant

The term “amino acid based surfactant” as used herein refers to achemical compound derived from a natural or synthetic amino acidmolecule and having surfactant properties. The amino acid basedsurfactants include fatty acid derivatives of amino acids, or saltsthereof, in which a fatty acid is attached to the amino acid throughcondensation of a carboxy group (—COOH) of the fatty acid and an aminogroup (—NH₂) of the amino acid to form an amide bond (—CONH—). Suitableamino acids may include, but are not limited to, glycine, lysine,sarcosine, glutamic acid, aspartic acid, aminomalonic acid, or anycombination thereof. Suitable fatty acids may include a C10-24 fattyacid, such as C12-20 or C12-18 fatty acids.

The term “C10-24 fatty acid” as used herein refers to a carboxylic acidshaving 1-8 carbon atoms. The carboxylic acid may have from 0 to 8carbon-carbon double bonds. The carboxylic acid may have a straight orbranched carbon chain, a cyclic carbon ring, or an aromatic carbon ring.The carboxylic acid may be unsubstituted or substituted with one or moresubstituent groups, each of which independently selected from hydroxy(—OH), amino, cyano, halogen, thiol, oxo (═O), and thioxo (═S). Examplesof suitable C10-24 fatty acids may include, but are not limited to,lauric acid, myristic acid, palmitic acid, stearic acid, behenic acid,myristoleic acid, oleic acid, linoleic acid, erucic acid, docosahexanoicacid, or any combination thereof.

In some embodiments, the amino acid based surfactant may comprise a010-24 fatty acid derivative of amino acid. In some further embodiments,the amino acid based surfactant may comprise a 010-24 fatty acidderivative of amino acid, wherein the amino acid comprises glycine,sarcosine, glutamic acid, aspartic acid, aminomalonic acid, orcombinations thereof.

Suitable amino acid based surfactants may include, but are not limitedto, sodium lauroyl sarcosinate, sodium lauroyl glycinate, sodium lauroylaminomalonate, sodium lauroyl aspartate, sodium N-lauroylglutamate,sodium cocoyl glutamate, or combinations thereof. Commercial examples ofamino acid based surfactants include sarcosinates marketed under thetradenames Perlastan® (Schill+Seilacher GmbH), Maprosyl® (StepanCompany, USA), Crodasinic® (Croda, UK), Oramix® (Seppic, France), andacylglutamate products, marketed as Amisoft-® (Ajinomoto Corp., Japan).

The disinfectant compositions may comprise at least 0.1%, at least 0.2%,at least 0.4%, at least 0.6%, at least 0.8%, at least 1.0%, at least2.0%, at least 2.5%, at least 3.0%, at least 3.5%, at least 4.0%, atleast 4.5%, at least 5.0%, at least 6.0%, at least 7.0%, at least 8.0%,at least 9.0%, at least 10.0%, at least 12.0%, at least 15.0%, at least20.0%, or at least 25.0% by weight of an amino acid based surfactant;and/or the compositions may comprise less than 30.0%, less than 25.0%,less than 20.0%, less than 15.0%, less than 12.0%, less than 10.0%, lessthan 9.0%, less than 8.0%, less than 7.0%, less than 6.0%, less than5.0%, less than 4.5%, less than 4.0%, less than 3.5%, less than 3.0%,less than 2.5%, less than 2.0%, less than 1.5%, less than 1.0%, lessthan 0.8%, less than 0.6%, less than 0.4%, or less than 0.2% by weightof an amino acid based surfactant. For example, the compositions maycomprise about 0.1% to about 30.0%, about 0.1% to about 25.0%, about0.1% to about 20.0%, about 0.1% to about 15.0%, about 0.1% to about10.0%, about 0.1 to about 8.0%, about 0.1% to about 6.0%, about 0.1% toabout 5.0%, about 0.1% to about 4.0%, about 0.1% to about 3.0%, about0.1% to about 2.0%, or about 0.1% to about 1.0% by weight of an aminoacid based surfactant. In some embodiments, the compositions compriseabout 0.1% to about 20.0% by weight of an amino acid based surfactant.In particular embodiments, the compositions comprise about 0.2% to about10.0% by weight, or about 0.2% to about 3.0% by weight of an amino acidbased surfactant.

Stabilizing Agent

The term “stabilizing agent” as used herein refers to an agent that mayhelp maintain the physical and chemical properties of the compositionsdisclosed herein. Suitable stabilizing agent for use in the discloseddisinfectant compositions may include, but are not limited to, C1-6alcohol, glycerol, polyol, glycol ether, or any combination thereof.Non-limiting examples of the C1-6 alcohols include methanol, ethanol,propanol, isopropanol, 1-butanol, 1-pentanol, 2-pentanol, 1-hexanol,2-hexanol, or any combination thereof. Non-limiting examples of thepolyols include alkane polyols having from 2-6 carbon atoms and from 2-3hydroxyls in the molecule. Non-limiting examples of the polyhydricalcohols include ethylene glycol, propylene glycol, dipropylene glycol,glycerin, 1,2-butanediol, 1,3-butanediol, 1,4-butanediol,2,3-butanediol, 1,2-propanediol, 1,5-pentanediol, meso-erythritol,neopentyl glycol, pentaerythritol, or any combination thereof. Examplesof the glycol ethers include, but are not limited to, those based onethylene or propylene glycol such as diethylene glycol ethyl ether,dipropylene glycol methyl ether, diethylene glycol methyl ether,diethylene glycol propyl ether, diethylene glycol butyl ether,ethylene/diethylene glycol 2-ethylhexyl ether, ethylene glycol phenylether, dipropylene glycol propyl ether, dipropylene glycol butyl ether,propylene glycol phenyl ether, or any combination thereof.

In some embodiments of the present disclosure, the disinfectantcomposition is substantially free of aromatic alcohol, e.g., benzylalcohol.

The compositions may comprise at least 0.05%, at least 0.1%, at least0.2%, at least 0.5%, at least 1.0%, at least 2.0%, at least 3.0%, atleast 4.0%, at least 5.0%, at least 6.0%, at least 7.0%, at least 8.0%,at least 9.0%, at least 10.0%, at least 12.0%, at least 15.0%, at least20.0%, or at least 25.0% by weight of a stabilizing agent; and/or thecompositions may comprise less than 30.0%, less than 25.0%, less than20.0%, less than 15.0%, less than 12.0%, less than 10.0%, less than9.0%, less than 8.0%, less than 7.0%, less than 6.0%, less than 5.0%,less than 4.0%, less than 3.0%, less than 2.0%, less than 1.0%, lessthan 0.5%, less than 0.2%, or less than 0.1% by weight of a stabilizingagent. For example, the composition may comprise about 0.05% to about30.0%, about 0.05% to about 25.0%, about 0.05% to about 20.0%, about0.05% to about 15.0%, about 0.05% to about 10.0%, about 0.05% to about8.0%, about 0.05% to about 6.0%, about 0.05% to about 4.0%, about 0.05%to about 2.0%, or about 0.05% to about 1.0% by weight of a stabilizingagent. In some embodiments, the compositions comprise about 0.1% toabout 20.0% by weight of a stabilizing agent. In particular embodiments,the compositions comprise about 1.0% to about 10.0% by weight, or about1.0% to about 6.0% by weight of a stabilizing agent.

Anionic Surfactant

Suitable anionic surfactants for use in the compositions may include,for example, alkyl sulfates, alkyl ether sulfates, alkyl arylsulfonates, alpha-olefin sulfonates, alkali metal or ammonium salts ofalkyl sulfates, alkali metal or ammonium salts of alkyl ether sulfates,alkyl phosphates, silicone phosphates, alkyl glyceryl sulfonates, alkylsulfosuccinates, alkyl taurates, acyl taurates, sulfoacetates, alkylphosphate esters, mono alkyl succinates, monoalkyl maleates,sulfoacetates, acyl isethionates, alkyl carboxylates, phosphate esters,sulfosuccinates (e.g., sodium dioctylsulfosuccinate), or any combinationthereof. Non-limiting examples of anionic surfactants may include sodiumlauryl sulfate, sodium lauryl ether sulfate, ammonium laurylsulfosuccinate, ammonium lauryl sulfate, ammonium lauryl ether sulfate,sodium dodecylbenzene sulfonate, triethanolamine dodecylbenzenesulfonate, sodium cocoyl isethionate, sodium lauroyl isethionate, or anycombination thereof.

In some embodiments, the disclosed disinfectant composition issubstantially free of linear benzyl benzene sulfonate, e.g., such asdodecylbenzenesulfonic acid.

The compositions may comprise at least 0.1%, at least 0.2%, at least0.4%, at least 0.6%, at least 0.8%, at least 1.0%, at least 2.0%, atleast 2.5%, at least 3.0%, at least 3.5%, at least 4.0%, at least 4.5%,at least 5.0%, at least 6.0%, at least 7.0%, at least 8.0%, at least9.0%, at least 10.0%, at least 12.0%, at least 15.0%, at least 20.0%, orat least 25.0% by weight of an anionic surfactant; and/or thecompositions may comprise less than 30.0%, less than 25.0%, less than20.0%, less than 15.0%, less than 12.0%, less than 10.0%, less than9.0%, less than 8.0%, less than 7.0%, less than 6.0%, less than 5.0%,less than 4.5%, less than 4.0%, less than 3.5%, less than 3.0%, lessthan 2.5%, less than 2.0%, less than 1.5%, less than 1.0%, less than0.8%, less than 0.6%, less than 0.4%, or less than 0.2% by weight of ananionic surfactant. For example, the compositions may comprise about0.1% to about 30.0%, about 0.1% to about 25.0%, about 0.1% to about20.0%, about 0.1% to about 15.0%, about 0.1% to about 10.0%, about 0.1to about 8.0%, about 0.1% to about 6.0%, about 0.1% to about 5.0%, about0.1% to about 4.0%, about 0.1% to about 3.0%, about 0.1% to about 2.0%,or about 0.1% to about 1.0% by weight of an anionic surfactant. In someembodiments, the compositions comprise about 0.1% to about 20.0% byweight of an anionic surfactant. In particular embodiments, thecompositions comprise about 0.2% to about 10.0% by weight, or about 0.2%to about 6.0% by weight of an anionic surfactant.

In some embodiments, the anionic surfactant may comprise an alkylsulfate, an alkyl ether sulfate, or a combination thereof. Suitablealkyl sulfates include, for example, sodium or ammonium salts of C12-14alcohol sulfate. Suitable alkyl ether sulfates include, for example,sodium or ammonium salts of C12-14 alcohol ethoxylate sulfate.

The compositions may comprise at least 0.1%, at least 0.2%, at least0.4%, at least 0.6%, at least 0.8%, at least 1.0%, at least 1.2%, atleast 1.4%, at least 1.6%, at least 1.8%, at least 2.0%, at least 2.2%,at least 2.4%, at least 2.6%, at least 2.8%, at least 3.0%, at least3.5%, at least 4.0%, at least 4.5%, at least 5.0%, at least 6.0%, atleast 7.0%, at least 8.0%, at least 9.0%, at least 10.0%, at least12.0%, or at least 14.0% by weight of an alkyl sulfate anionicsurfactant; and/or the compositions may comprise less than 15.0%, lessthan 14.0%, less than 12.0%, less than 10.0%, less than 9.0%, less than8.0%, less than 7.0%, less than 6.0%, less than 5.0%, less than 4.5%,less than 4.0%, less than 3.5%, less than 3.0%, less than 2.8%, lessthan 2.6%, less than 2.4%, less than 2.2%, less than 2.0%, less than1.8%, less than 1.6%, less than 1.4%, less than 1.2%, less than 1.0%,less than 0.8%, less than 0.6%, less than 0.4%, or less than 0.2% byweight of an alkyl sulfate anionic surfactant. For example, thecompositions may comprise about 0.1% to about 15.0%, about 0.1% to about12.0%, about 0.1% to about 10.0%, about 0.1% to about 9.0%, about 0.1 toabout 8.0%, about 0.1% to about 7.0%, about 0.1% to about 6.0%, about0.1% to about 5.0%, about 0.1% to about 4.0%, about 0.1% to about 3.0%,about 0.1% to about 2.5%, about 0.1% to about 2.0%, about 0.1% to about1.5%, or about 0.1% to about 1.0% by weight of an alkyl sulfate anionicsurfactant. In some embodiments, the compositions comprise about 0.2% toabout 10.0% by weight or about 0.2% to about 3.0% by weight of an alkylsulfate anionic surfactant.

The composition may comprise at least 0.1%, at least 0.2%, at least0.4%, at least 0.6%, at least 0.8%, at least 1.0%, at least 1.2%, atleast 1.4%, at least 1.6%, at least 1.8%, at least 2.0%, at least 2.2%,at least 2.4%, at least 2.6%, at least 2.8%, at least 3.0%, at least3.5%, at least 4.0%, at least 4.5%, at least 5.0%, at least 6.0%, atleast 7.0%, at least 8.0%, at least 9.0%, at least 10.0%, at least12.0%, or at least 14.0% by weight of an alkyl ether sulfate anionicsurfactant; and/or the compositions may comprise less than 15.0%, lessthan 14.0%, less than 12.0%, less than 10.0%, less than 9.0%, less than8.0%, less than 7.0%, less than 6.0%, less than 5.0%, less than 4.5%,less than 4.0%, less than 3.5%, less than 3.0%, less than 2.8%, lessthan 2.6%, less than 2.4%, less than 2.2%, less than 2.0%, less than1.8%, less than 1.6%, less than 1.4%, less than 1.2%, less than 1.0%,less than 0.8%, less than 0.6%, less than 0.4%, or less than 0.2% byweight of an alkyl ether sulfate anionic surfactant. For example, thecompositions may comprise about 0.1% to about 15.0%, about 0.1% to about12.0%, about 0.1% to about 10.0%, about 0.1% to about 9.0%, about 0.1 toabout 8.0%, about 0.1% to about 7.0%, about 0.1% to about 6.0%, about0.1% to about 5.0%, about 0.1% to about 4.0%, about 0.1% to about 3.0%,about 0.1% to about 2.5%, about 0.1% to about 2.0%, about 0.1% to about1.5%, or about 0.1% to about 1.0% by weight of an alkyl ether sulfateanionic surfactant. In some embodiments, the compositions comprise about0.2% to about 10.0% by weight, or about 0.2% to about 3.0% by weight ofan alkyl ether sulfate anionic surfactant.

Oxidizing Agent

The compositions disclosed herein may optionally include an oxidizingagent. Suitable oxidizing agents may include, for example, ammoniumpersulfate, sodium persulfate, potassium persulfate, hydrogen peroxide,tert-butyl hydroperoxide, cumene hydroperoxide, tertamyl hydroperoxide,tert-butylperpivalate, tert-butyl perbenzoate, benzoyl peroxide,peroxyacids (like peracetic acid), ozone, chlorine dioxide, or anycombination thereof. In some embodiments, the oxidizing agent may be aperoxide such as hydrogen peroxide.

The compositions may comprise at least 0.01%, at least 0.05%, at least0.1%, at least 0.2%, at least 0.3%, at least 0.4%, at least 0.5%, atleast 1.0%, at least 2.0%, at least 3.0%, or at least 4.0% by weight ofan oxidizing agent; and/or the compositions may comprise less than 5.0%,less than 4.0%, less than 3.0%, less than 2.0%, less than 1.5%, lessthan 1.0%, less than 0.5%, less than 0.4%, less than 0.3%, less than0.2%, or less than 0.1% by weight of an oxidizing agent. For example,the compositions may comprise 0.01% to about 5.0%, about 0.01% to about4.0%, about 0.01% to about 3.0%, about 0.01% to about 2.0%, about 0.01%to about 1.5%, about 0.01% to about 1.0%, about 0.01% to about 0.5%,about 0.01% to about 0.4%, about 0.01% to about 0.3%, about 0.01% toabout 0.2%, or about 0.01% to about 0.1% by weight of an oxidizingagent. In some embodiments, the compositions comprise about 0.01% toabout 1.5% by weight, or about 0.5% to about 1.5% by weight of anoxidizing agent.

In some embodiments, the composition comprises an oxidizing agent andthe oxidizing agent is peroxide, which is present at about 0.5% to about1.5% by weight.

In some embodiments, the composition is substantially free of anyoxidizing agent. In some embodiments, the composition includes theoxidizing agent in an amount of less than 0.005%, less than 0.001%, lessthan 0.0005%, or less than 0.0001% by weight.

In some embodiments, the compositions as disclosed herein have a pH of 5or less. The compositions may have a pH value of less than 4.5, lessthan 4.0, less than 3.5, less than 3.0, less than 2.5, less than 2.0,less than 1.5, or even less than 1.0; and/or the compositions may have apH value of at least 0.1, at least 0.5, at least 1.0, at least 1.5, atleast 2.0, at least 2.5, at least 3.0, at least 3.5, at least 4.0, or atleast 4.5. In some embodiments, the pH is about 1.0 to about 3.0,including about 1.5 to about 3.0, about 2.0 to 3.0, about 2.1 to 3.0,about 2.2 to 3.0, about 2.2 to 3.0, about 2.3 to 3.0, about 2.4 to 3.0,or about 2.5 to 3.0. In particular embodiments, the pH is about 2.0 toabout 3.0, including about 2.1 to about 2.9, about 2.2 to 2.8, or about2.3 to 2.8.

The composition as disclosed herein may further include an effectiveamount of one or more additional ingredients selected from pH adjustingagents, buffering agents, nonionic surfactants, hydrotropes, corrosioninhibitors, sequestering agents, antimicrobial compounds, dyes, rheologymodifiers, preservatives, moisturizing agents, emollients, or anycombination thereof. The compositions may comprise about 0.01% to about5.0%, such as about 0.01% to about 5.0%, about 0.01% to about 4.0%,about 0.01% to about 3.0%, about 0.01% to about 2.0%, about 0.01% toabout 1.0%, about 0.01% to about 0.5%, or about 0.01% to about 0.1% byweight each of one or more of the additional ingredients.

Suitable pH adjusting agents include substances demonstrating analkaline property or substances demonstrating an acidic property.Examples of substances demonstrating an alkaline property include, butare not limited to, alkali metal hydroxides such as sodium hydroxide andpotassium hydroxide; carbonates such as sodium carbonate, sodiumbicarbonate, and potassium carbonate; silicates such as sodium silicateand potassium silicate; borates such as sodium borate; organic acidsalts such as sodium citrate; amines such as monoethanolamine anddiethanolamine, and ammonia. Examples of substances that demonstrate anacidic property and used for pH adjustment include, but are not limitedto, inorganic acids such as hydrochloric acid and sulfuric acid, ororganic acids such as citric acid and acetic acid.

Suitable buffering agents may include, but are not limited to,phosphoric acid, monosodium phosphate, disodium phosphate, trisodiumphosphate, citric acid and salts such as sodium citrate, benzoic acid,sodium benzoate, or any combination thereof.

Suitable nonionic surfactant may include, but are not limited to,ethylene oxide adducts of C8 to C22, preferably C8 to C16, morepreferably C8 to C12 alcohols; ethylene oxide/propylene oxide adducts ofethylene glycol; alkylene glycols; alkyl polyglucosides; or mixturesthereof. Non-limiting examples of suitable types of non-ionic surfactantinclude ethoxylates of alkyl polyethylene glycol ethers, polyalkyleneglycol (e.g., 100% Breox FCC92), or alcohol alkoxylate EO/PO (e.g.,Plurafac LF403). Exemplary alcohol ethoxylates include fatty alcoholethoxylates, e.g., tridecyl alcohol alkoxylate, ethylene oxide adduct,alkyl phenol ethoxylates, or ethoxy/propoxy block surfactants. In someembodiments, the nonionic surfactant is alcohol ethoxylates, EO/PO blockcopolymers, alkyl polyglucosides, or any combination thereof.

Suitable hydrotropes may include, but are not limited to, benzenesulfonates, naphthalene sulfonates, alkyl benzene sulfonates,naphthalene sulfonates, alkyl sulfonates, alkyl sulfates, alkyldiphenyloxide disulfonates, or phosphate ester hydrotropes. Exemplaryalkyl benzene sulfonates include, for example, isopropylbenzenesulfonates, xylene sulfonates, toluene sulfonates, cumene sulfonates, ormixtures any two or more thereof. Exemplary alkyl sulfonates includehexyl sulfonates, octyl sulfonates, and hexyl/octyl sulfonates, ormixtures of any two or more thereof. In some embodiments, the hydrotropecomprises sodium xylene sulfonate, sodium cumene sulfonate, orcombinations thereof.

Suitable corrosion inhibitors may include, but are not limited to,triazoles such as benzotriazole, tolyltriazole, andmercaptobenzothiazole; gluconate salts such as sodium gluconate,potassium gluconate and ammonium gluconate; molybdate salts such assodium molybdate, or any combination thereof.

Suitable sequestering agents may include, but are not limited to,various known phosphate and non-phosphate builder materials.Non-limiting examples of suitable non-phosphate agents include alkalimetal citrates; carbonates; bicarbonates; the salts of nitrilotriaceticacid (NTA), methylglycine diacetic acid (MGDA), or glutaric diaceticacid (GLDA); polycarboxylates such as polymaleates, polyacetates,polyhydroxyacrylates, polyacrylate/polymaleate andpolyacrylate/polymethacrylate copolymers; zeolites, layered silica; ormixtures thereof. In some embodiments, the sequestering agent isselected from phosphates, NTA, EDTA, MGDA, GLDA, citrates, carbonates,bicarbonates, polyacrylate/polymaleate, or maleicanhydride/(meth)acrylic acid copolymers, e.g., Sokalan CP5 availablefrom BASF.

The sequestering agent herein may also include anti-scalants, such aspolyacrylates of molecular weight from 1,000 to 400,000, examples ofwhich are supplied by Rohm & Haas, BASF and Alco Corp., or polymersbased on acrylic acid combined with other moieties. These includeacrylic acid combined with maleic acid, such as Sokalan CP5 and CP7supplied by BASF or Acusol 479N supplied by Rohm & Haas with methacrylicacid such as Colloid 226/35 supplied by Rhone-Poulenc; with phosphonatesuch as Casi 773 supplied by Buckman Laboratories; with maleic acid andvinyl acetate such as polymers supplied by Huls; with acrylamide; withsulfophenol methallyl ether such as Aquatreat AR 540 supplied by Alco;with 2-acrylamido-2-methylpropane sulfonic acid such as Acumer 3100supplied by Rohm & Haas or such as K-775 supplied by Goodrich; with2-acrylamido-2-methylpropane sulfonic acid and sodium styrene sulfonatesuch as K-798 supplied by Goodrich; with methyl methacrylate, sodiummethallyl sulfonate and sulfophenol methallyl ether such as Alcosperse240 supplied by Alco; polymaleates such as Belclene 200 supplied by FMC;polymethacrylates such as Tamol 850 from Rohm & Haas polyaspartates;ethylenediamine disuccinate; or organo polyphosphonic acids and theirsalts such as the sodium salts of aminotri(methylenephosphonic acid) andethane-1-hydroxy-1,1-diphosphonic acid. In some embodiments, thesequestering agent may be phosphate, phosphonates, EDTA, MGDA, GLDA,polyacrylates, or any combination thereof.

Suitable antimicrobial compounds may include agents that can kill orotherwise inhibit the growth or proliferation of microbes including, forexample, bacteria, viruses or fungi. Examples of antimicrobial compoundsinclude, but are not limited to, quaternary ammonium salts, essentialoils, antimicrobial pesticides, antibacterials, antivirals, orantiparasitics known in the art.

The compositions may contain dyes used in disinfectant products tovisualize coverage or pH sensitive dyes such as thymolphthalein,bromothyol blue, and phenol phenolphthalein.

Suitable rheology modifiers may include, but are not limited to, watersoluble cellulosic polymers such as hydroxyethyl cellulose orhydroxypropyl cellulose.

Preservatives may include, but are not limited to, antimicrobials,biocides, or salts. For example, preservatives may include, but are notlimited to, KATHON™ Preservatives (mixtures includingmethylchloroisothiazolinone and methylisothiazolinone from Dow Chemical,Midland, Mich.); NEOLONE 950™ (methylisothiazolinone from Dow Chemical,Midland, Mich.); DMDM hydantoin (available as GLYDANT™ from Lonza,Allendale, N.J.); iodopropynyl butylcarbamate; formaldehyde; benzoicesters (parabens), such as methylparaben, propylparaben, butylparaben,ethylparaben, isopropylparaben, isobutylparaben, benzylparaben;2-bromo-2-nitropropane-1,3-diol; benzoic acid; formic acid; or thoseknown to those skilled in the art.

The disclosed disinfectant compositions may include at least one skinconditioner such as an emollient, humectant, occlusive agent, or othermoisturizing agent to provide moisturizing, skin softening, skin barriermaintenance, anti-irritation, or other skin health benefits. Somenon-limiting examples of emollients include stearoxytrimethylsilane,alkyl benzoate, silicone oils, dimethicone, myristyl myristate, cetylmyristate, glyceryl dioleate, methyl laurate, PPG-9 laurate, octylpalmitate, lanolin, propylene glycol, glycerol, fatty acids, naturaloils such as sunflower, almond, mineral, canola, sesame, soybean, wheatgerm, corn, peanut and olive, isopropyl myristate, myristyl alcohol,aloe vera, hydrolyzed silk protein, Vitamin E, stearyl alcohol,isopropyl palmitate, sorbitol, amino acid complexes, or polyethyleneglycol. Some nonlimiting examples of humectants include hydroxyethylurea, agarose, arginine PCA, fructose, glucose, glutamic acid, glycerol,honey, lactose, maltose, propylene glycol, polyethylene glycol,sorbitol, or any mixtures thereof. Some non-limiting examples ofocclusive agents include petrolatum, shea butter, alkyl dimethicones,avocado oil, balm mint oil, canola oil, cod liver oil, corn oil,methicone, mineral oil, olive oil, phenyl trimethicone, trimyristin,soybean oil, glycol distearate, stearyl stearate, synthetic wax, ormixtures thereof. Some non-limiting examples of other moisturizersinclude cholesterol, cystine, hyaluronic acid, keratin, lecithin, eggyolk, glycine, PPG-12, panthenol, retinol, vegetable oil, or anymixtures thereof. Some non-limiting examples of anti-irritants includebisabolol or panthenol.

In general, the disinfectant compositions as disclosed herein may beprepared by thoroughly mixing the ingredients at room temperature with amixer or blender such that a homogeneous mixture is obtained.Commercially available ingredients are typically used without furtherpurification or treatment. For liquid compositions, the ingredients maybe dissolved in a solvent or liquid carrier (such as alcohol or water)to form a solution. Individual ingredients may be added during thepreparation process in any order.

The disclosed disinfectant compositions may have antimicrobialactivities measurable by assay methods known in the art, such as thosedescribed in “Guidance on the Biocidal Products Regulation, Volume IIEfficacy—Assessment and Evaluation (Parts B+C)” (version 3.0, April2018) and “Performance Criteria—Overview of (EN) Standards, TestConditions, and Pass Criteria” (Appendix 4, BPR Efficacy Working GroupDocument, March 2017), both available at European Chemical Agency(ECHA), the entire contents of each of which are incorporated herein byreference. In some embodiments, the disclosed compositions have anantimicrobial activity of log reduction of at least 2 under BiocidalProduct Registration (BPR) standard EN 13727, EN 13624, or EN 1499. Forexample, the antimicrobial activity may achieve a log reduction of atleast 2.0, at least 2.5, at least 3.0, at least 3.5, at least 4.0, atleast 4.5, at least 5.0, at least 5.5, or at least 6.0 under BPRstandard EN 13727, EN1276, EN13624, or EN1499. This includes anantimicrobial activity of log reduction of about 2.5, about 3.0, about3.5, about 4.5, about 5.0, about 5.5, or about 6.0. The antimicrobialactivity may be in the range of log reduction of about 2.0 to about 6.0,about 2.0 to about 5.0, about 2.0 to about 4.0, about 2.0 to about 3.0,about 3.0 to about 6.0, about 3.0 to about 5.0, about 3.0 to about 4.0,about 4.0 to about 6.0, or about 4.0 to about 5.0. In particularembodiments, the compositions have an antimicrobial activity of logreduction of at least 2.0, at least 3.0, or at least 4.0 under the BPRstandard tests. Other suitable measurement standards for antimicrobialactivity include those described in the regulatory guidelines of UnitedStates Environmental Protection Agency (EPA), such as the EPA standardthat applies the “Quantitative Methods for Evaluating the Activity ofMicrobicides used on Hard, Non-Porous Surface” issued by theOrganisation for Economic Co-operation and Development (OECD)(hereinafter “the OECD Quantitative Methods for Evaluating the Activityof Microbicides”), or “Series 810 —Product Performance Test Guidelines”,as well as those required by the health agencies in other countries. Thedisclosed compositions may satisfy the BPR standards, the EPA standardaccording to the OECD Quantitative Methods for Evaluating the Activityof Microbicides, and/or other standards intended to measureantimicrobial activity at a similar level using a similar testingprocedure.

Conventional organic acid based antimicrobial actives that are typicallyused in liquid disinfectant often present limitations in reachingantimicrobial efficacies at the permitted levels. Advantageously, thepresent disclosure provides a disinfectant composition, in which theantimicrobial efficacy is enhanced through the synergy between C1-8organic acids and amino acid based surfactants. As a result, thedisclosed disinfectant compositions may achieve a desirabale level ofantimicrobial activity with a reduced amount of the antimicrobialactives, comparable to the currently available commercializeddisinfectant products.

The disclosed disinfectant compositions may provide an enhancedantimicrobial efficacy, at least, through the unexpected synergisticeffect between C1-8 organic acid and amino acid based surfactant. See,e.g., EXAMPLES 1-6 and 9. This unexpected synergy between C1-8 organicacid and amino acid based surfactant could be achieved against variousmicroorganisms, such as bacteria, yeasts, fungi, spores, viruses, etc.As non-limiting examples, the disclosed disinfectant compositions mayprovide a synergistic micro efficacy against the followingmicroorganism: Staphylococcus aureus, Mycobacterium smegmatis, Candidaalbicans, Mycobacterium bovis, Trychophyton interdigitale, and the like.See, e.g., EXAMPLES 1-3 for S. aureus; EXAMPLES 4, 5 and 11 for M.smegmatis; EXAMPLES 6 and 9 for C. albicans; EXAMPLE 10 for T.interdigitale. Such an enhanced micro efficacy of the discloseddisinfectant compositions may be achieved even without adding oxidizingagent (such as hydrogen peroxide) in the compositions. See, e.g.,EXAMPLES 1, 3, 5, 6, 8 and 9. Upon including an oxidizing agent, such ashydrogen peroxide, into the disclosed disinfected compositions, afurther enhanced antimicrobial efficacy could be achieved. As anon-limiting example, EXAMPLE 9 shows that upon including hydrogenperoxide into the disinfectant composition (along with C1-18 organicacid, amino acid based surfactant, and stabilizing agent), the amount ofC1-8 organic acid required to achieve the desirable micro efficacy couldbe reduced.

In some embodiments of the present disclosure, the disinfectantcompositions may comprise a C1-8 organic acid, an amino acid surfactant,and a stabilizing agent. In some embodiments, the disclosed disinfectantcompositions may comprise a C1-8 organic acid, an amino acid surfactant,a stabilizing agent, and an oxidizing agent (e.g., hydrogen peroxide).

The combination of antimicrobial actives (such as the organic acids usedin liquid disinfectant solutions) with surface active ionic species(specifically amino acid based surfactants) generally presentsformulation stability challenges at the levels sufficient to achieve aneffective formulation. Significantly, the stability challenges alsoextend to the formulations involving oxidizing agents, such as peroxidesand the like used in conventional products. Advantageously, thedisclosed disinfectant compositions include a stabilizing agent toachieve the physical stability with desired shelf life, withoutinterfering with the synergistic effect between amino acid basedsurfactants and C1-8 organic acids. Thus, the disclosed disinfectantcompositions may provide both enhanced antimicrobial activity andimproved stability with reduced concentration of active ingredients andand/or without the use of oxidizing agents. As non-limiting examples,EXAMPLES 7 and 10 show that the physical stability of the discloseddisinfectant compositions may be significantly improved due to thepresence of stabilizing agent in the compositions. Moreover, thedisclosed disinfectant compositions may maintain the same antimicrobialactivity even after at least one month at 40° C. storage. See EXAMPLE 8.

In some embodiments, the disclosed disinfectant compositions may havesubstantially the same antimicrobial activity after at least one monthat 40° C. storage. In some embodiments, the disclosed disinfectantcompositions may have substantially the same antimicrobial activity evenafter at least 3 months at 40° C. storage.

The disclosed disinfectant compositions may be physically and chemicallystable during storage. For example, the composition may be in the formof a liquid solution which maintains its physical appearance as a clearsolution without detectable phase separation, precipitation, coloration,or crystallization of the solute during storage. The solution maymaintain a constant pH or undergo minimum extent of degradation orchemical change in its ingredients including, for example, a degradationof less than 1.0%, less than 0.5%, less than 0.1%, less than 0.05%, oreven 0.01% of its active ingredients. The duration of the storage, forexample, may be about one month, about two months, about three months,about six months, about nine months, or even about twelve months.

The disclosed composition may also maintain substantially the same levelof chemical and biological activities over the storage time. Theproperty to “maintain” an antimicrobial activity as used herein means aproduct's ability to retain its antimicrobial activity at a level thatis at least 90% of its original activity when the product is freshlyprepared. For example, the present compositions may maintain at least90%, at least 91%, at least 92%, at least 93%, at least 94%, at least95%, at least 96%, at least 97%, at least 98%, at least 99%, at least99.5%, or even at least 99.9% of their antimicrobial activities over atime period of at least two weeks, at least one month, at least twomonths, at least three months, or even at least 6 months. Thecompositions may maintain about 90%, about 91%, about 92%, about 93%,about 94%, about 95%, about 96%, about 97%, about 98%, about 99%, about99.5%, or even about 99.9% of the antimicrobial activity over a periodof at least one month or at least three months. In some embodiments, thecompositions maintain about 90%, about 91%, about 92%, about 93%, about94%, about 95%, about 96%, about 97%, about 98%, about 99%, about 99.5%,or even about 99.9% of the antimicrobial activity over a period of atleast three months.

The present compositions may be stable over a temperature range of about0° C. to about 50° C. including for example, about 0° C. to about 45°C., about 0° C. to about 40° C., about 0° C. to about 35° C., about 0°C. to about 30° C., about 0° C. to about 25° C. (or room temperature),or about 0° C. to about 20° C. storage. For example, the compositionsmay maintain their antimicrobial activities at a temperature of about 0°C., about 5° C., about 10° C., about 15° C., about 20° C., about 25° C.,about 30° C., about 35° C., about 40° C., about 45° C., or even about50° C. storage for a period of at least two weeks, at least one month,at least two months, at least three months, or even at least six months.In some embodiments, the compositions maintain about 90%, about 91%,about 92%, about 93%, about 94%, about 95%, about 96%, about 97%, about98%, about 99%, about 99.5%, or even about 99.9% of the antimicrobialactivity at a temperature range of about 0° C. to about 40° C. storageover a period of at least one month or at least three months. In someembodiments, the compositions maintain about 90%, about 91%, about 92%,about 93%, about 94%, about 95%, about 96%, about 97%, about 98%, about99%, about 99.5%, or even about 99.9% of the antimicrobial activity at atemperature of about 0° C., about 25° C., or about 40° C. storage over aperiod of at least one month. In some embodiments, the compositionsmaintain about 90%, about 91%, about 92%, about 93%, about 94%, about95%, about 96%, about 97%, about 98%, about 99%, about 99.5%, or evenabout 99.9% of the antimicrobial activity at a temperature of about 0°C., about 25° C., or about 40° C. storage over a period of at leastthree months.

The stabilizing agent may contribute to an increase in the antimicrobialactivity of the disclosed disinfectant compositions. At least, this maybe due to the unexpected synergistic effect between the amino acid basedsurfactant and the stabilizing agent. See EXAMPLE 11.

In some embodiments, the disclosed disinfectant composition is in theform of a solid, such as a powder or a tablet, or a liquid, such as asolution or a suspension. In some embodiments, the composition is powerthat may be dissolved by a user with a suitable solvent (such as water)to prepare a liquid disinfectant. In some embodiments, the compositionis a ready-to-use solution. In some embodiments, the composition is aliquid concentrate, which may be diluted by a user with a suitablediluent at a concentrate/diluent ratio of, for example, 1:1 to 1:1000 byweight. This includes a concentrate/diluent ratio of about 1:5, about1:10, about 1:20, about 1:50, about 1:100, about 1:200, or about 1:500by weight.

It has been known in the art that the concentrated disinfectantcomposition having an amount of salicylic acid above 0.2% weight, basedon total weight of the composition, is rather difficult to achieve. Thisis due to the poor solubility of salicylic acid at such concentratedamount, thereby generally resulting on a phrase separation of theformulation. Surprisingly, the disinfectant composition of presentdisclosure could be obtained at high level of salicylic acid (e.g.,above 0.2% weight based on total weight of the disinfectantcomposition), with high stability and excellent antimicrobial efficacy.See EXAMPLE 10.

The disinfectant compositions disclosed herein may be built onplant-origin, biodegradable raw materials and demonstrate desiredproduct attributes such as the foam profile comparable to commercialnon-green product, at the same time providing enhanced antimicrobialefficacy. The compositions disclosed herein may have improved olfactoryaspects compared to conventional disinfectant products that containbiodegradable and/or plant origin raw materials. For example, thecompositions may be odorless or essentially free of any unpleasant odor.In some embodiments, the compositions disclosed herein may not produceany negative or unsatisfactory olfactory aspects and are thereforelikely to be used more frequently, providing increased sanitizationcompliance (e.g., hand washing).

In some embodiments, the disclosed synergistic disinfectant compositioncomprises:

-   -   a C1-8 organic acid;    -   an amino acid based surfactant; and    -   a stabilizing agent,    -   wherein the composition has an antimicrobial activity of log        reduction of at least 2 under Biocidal Product Registration        (BPR, Regulation (EN) 528/2012) standard EN13727, EN1276,        EN13624, or EN1499; and/or the EPA standard according to the        OECD Quantitative Methods for Evaluating the Activity of        Microbicides, and    -   wherein the composition has substantially the same antimicrobial        activity after at least one month at 40° C. storage.

In some embodiments, the disinfectant composition may further comprisean oxidizing agent.

In some embodiments, the disinfectant composition may further comprisean anionic surfactant.

In some embodiments, the disclosed synergistic disinfectant compositioncomprises:

-   -   from about 0.1% to about 25.0% by weight of a C1-8 organic acid;    -   from about 0.1% to about 20.0% by weight of an amino acid based        surfactant;    -   from about 0.1% to about 20.0% by weight of an anionic        surfactant; and    -   from about 0.05% to about 20.0% by weight of a stabilizing        agent,    -   all based on total weight of the disinfectant composition.

In some embodiments, the disinfectant composition may further comprisefrom about 0.01% to about 5.0% by weight of an oxidizing agent based ontotal weight of the composition.

In some embodiments, the balance of the composition is water.

In some embodiments, the disclosed disinfectant composition issubstantially free of linear benzyl benzene sulfonate (e.g.,dodecylbenzenesulfonic acid) and/or aromatic alcohol (e.g., benzylalcohol).

In another aspect, the present disclosure provides a method ofdisinfecting a surface, the method comprising applying a synergisticdisinfectant composition as disclosed herein to the surface. Suitablesurfaces that may be disinfected using the present compositions include,but are not limited to metal, ceramic, glass, plastic, stainless steel,or wood surfaces. Metal surfaces may include soft metal surfaces, suchas aluminum or alloy surfaces. The surface may be a part of a ware, aninstrument, a device, furniture, upholstery, or an operational platformused in household, restaurant, healthcare facilities, or other publicareas. Examples of household surfaces including, but are not limited to,dishes, cooking wares, cooking devices or appliances, tables, stairs,floors, tiles, sinks, and toilets.

The disclosed synergistic compositions may also be used to disinfect abody surface of an animal, including human, cat, dog, or other animals.For example, the composition may be used to disinfect the surface of ahuman's hand, leg, or other body parts. In some embodiments, thecompositions may be used to disinfect human hands.

The following non-limiting examples illustrate the compositions of thepresent disclosure and methods of use thereof.

EXAMPLES

Materials and Methods

Commercially available materials include: alkyl (C12-14) 2E0 sulphuricacid Na-salt (Texapon® N 70, BASF; Emal® 228 HP, KAO; Rhodapex® ESB 70NAT, Solvay), sodium dodecyl sulphate (Galaxy 780, Galaxy surfactantsLtd; Calfoam® SLS-30, Pilot; Rhodapon® LS 92RNB, Solvay), N-lauroylsarcosinic acid Na-salt (Crodasinic™ LS30-LQ-(RB), Croda; Perlastan®L30, Schill & Seilacher), lactic acid (Lactic Acid 88% FCC, CorbionPurac; Galacid Excel 88, Jungbunzlauer), citric acid (Citric Acid,monohydrate, Hangzhou Ruijiang or Jungbunzlauer), glycolic acid(Clariant, Merck).

General preparation methods. The experimental batches were prepared inlab using the listed ingredients with stepwise addition of each rawmaterial and mixing using a steel blade stirrer at 200 to 300 rpm. Thebalance water was maintained so that the total composition of theresultant homogeneous mixture was at 100%.

Instruments used and general testing methods. The batches were testedfor physical parameters using standard testing methods. Specific gravitywas measured under ASTM D1217 using pycnometer. The pH values weremeasured using ASTM E70-07 (pH meter using glass electrode).

Antimicrobial activities were determined according to the regulatoryguidelines of United States Environmental Protection Agency (EPA),according to the OECD Quantitative Methods for Evaluating the Activityof Microbicides.

Furthermore, the antimicrobial activities were determined under theBiocidal Products Regulation (BPR) standard EN13727, EN13624, or EN1499according to “Performance Criteria—Overview of (EN) Standards, TestConditions, and Pass Criteria” (Appendix 4, BPR Efficacy Working GroupDocument, March 2017) available at European Chemical Agency (ECHA).

The term “log reduction” is a mathematical term used to show therelative number of live microbials being reduced from a tested area. Forexample, “a log reduction of 5” or “a 5-log reduction” means loweringthe number of microbials by 105; “a 4-log reduction” means lowering thenumber of microbials by 104; “a 3-log reduction” means lowering thenumber of microbials by 103; “a 2-log reduction” means lowering thenumber of microbials by 102; and “a 1-log reduction” means lowering thenumber of microbials by 10.

Example 1

Table 1 showed the micro efficacy of the tested disinfectantformulations against S. aureus based on the EPA standard according tothe OECD Quantitative Methods for Evaluating the Activity ofMicrobicides. The organic acids were a mixture of salicylic acid andlactic acid. The amino acid based surfactant was sodium cocoyl glutamate(hereinafter “sodium glutamate”), and the stabilizing agent waspropylene glycol n-butyl ether (hereinafter “PnB”).

TABLE 1 Formulation #1 #2 #3 Ingredients On 100% On 100% On 100%Salicylic acid 0 0.08 0.08 Lactic acid 0 0.38 0.38 Hydrogen peroxide 1 11 Sodium glutamate 1 0 1 PnB 3 3 3 Sodium xylene sulfonate 0.3 0.3 0.3Water Bal. Bal. Bal. Micro Efficacy against 2.02 0.54 3.66 S. aureus(Log Reduction)

Formulation #1, which contained C1-8 organic acids but not amino acidbased surfactant, provided a 2.02-log reduction against S. aureus.Formulation #2, which contained amino acid based surfactant but not C1-8organic acids, provided a 0.54-log reduction. Formulation #3, whichcontained both C1-8 organic acids and amino acid based surfactant,provided a 3.66-log reduction against S. aureus.

If the effect between C1-8 organic acids and sodium glutamate surfactantwas merely an additive effect, the expected log reduction of S. aureusfor Formulation #3 (contained both amino acid based surfactant andorganic acids) would be 10^(2.02)+10^(0.54)=10^(2.03), which would showan expected log reduction of 2.03.

However, Formulation #3 showed a log reduction of 3.66 against S.aureus, which is larger than the additive log redutions from Formulation#1 and Formulation #2. Therefore, there was a synergistic effect betweenC1-8 organic acids and amino acid based surfactant against S. aureus.

Example 2

Table 2 showed the synergistic effect between organic acids (a mixtureof salicylic acid and lactic acid) and amino acid based surfactant(sodium glutamate) against S. aureus under the EPA standard according tothe OECD Quantitative Methods for Evaluating the Activity ofMicrobicides.

TABLE 2 Formulation #4 #5 #6 #7 Ingredients On 100% On 100% On 100% On100% Salicylic acid 0.15 0 0.15 0.15 Lactic acid 0.75 0 0.75 0.75Hydrogen peroxide 1 1 1 0 Sodium glutamate 0 1.5 1.5 1.5 PnB 3 3 3 3Sodium xylene 0.3 0.3 0.3 0.3 sulfonate Water Bal. Bal. Bal. Bal. MicroEfficacy against 5.03 2.95 6.38 6.38 S. aureus (Log Reduction)

Formulation #4, which contained C1-8 organic acids but not sodiumglutamate, provided a 5.03-log reduction against S. aureus. Formulation#5, which contained sodium glutamate but not C1-8 organic acids,provided a 2.95-log reduction against S. aureus. Formulation #6, whichcontained both C1-8 organic acids and sodium glutamate, provided a6.38-log reduction against S. aureus.

If the effect between C1-8 organic acids and sodium glutamate surfactantwas merely an additive effect, the expected log reduction of S. aureusfor Formulation #6 (contained both amino acid based surfactant andorganic acids) would be 10^(5.03)+10^(2.95)=10^(5.034), which would showan expected log reduction of 5.034.

However, Formulation #6 showed a log reduction of 6.38 against S.aureus, which is larger than the additive log redutions from Formulation#4 and Formulation #5. Therefore, there was a synergistic effect betweenC1-8 organic acids and amino acid based surfactant against S. aureus.

Formulation #7 contained the same ingredients as Formulation #6, butwithout hydrogen peroxide (an oxidizing agent). The micro efficacy S.aureus of Formulation #7 was about the same as Formulation #6. Thus,such high efficacy against S. aureus was achieved even without the useof hydrogen peroxide in the formulation.

Example 3

Table 3 showed the micro efficacy of the tested disinfectantformulations against S. aureus based on the EPA standard according tothe OECD Quantitative Methods for Evaluating the Activity ofMicrobicides.

TABLE 3 Formulation A B C D Ingredients On 100% On 100% On 100% On 100%C1-8 Organic acids 0 2.6 2.6 2.6 Hydrogen peroxide 0.5 0.5 0.5 0 Sodiumsarcosinate 1.5 0 1.5 1.5 Ethanol 5 5 5 5 Sodium xylene 0.3 0.3 0.3 0.3sulfonate Water Bal. Bal. Bal. Bal. Micro Efficacy 3.29 0.82 6.38 6.38against S. aureus (Log Reduction)

A very strong synergistic effect between C1-8 organic acids and aminoacid based surfactant against S. aureus was observed in the disinfectantFormulation C, wherein the organic acids were a mixture of salicylicacid and lactic acid (at 0.4% weight and 2.2% weight, respectively,based on total weight of the formulation), the amino acid basedsurfactant was a sodium salt of N-lauroyl sarcosinate (hereinafter“Sodium sarcosinate”), and the stabilizing agent was ethanol.Formulation D showed that the high efficacy against S. aureus wereachieved even without the use of hydrogen peroxide in the formulation.

Example 4

M. smegmatis was known as the surrogate strain for M. tuberculosis. Themicro efficacy of the disinfectant formulation was determined based onthe EPA standard according to the OECD Quantitative Methods forEvaluating the Activity of Microbicides. Table 4 demonstrated thesynergistic effect between C1-8 organic acids and amino acid basedsurfactant against M. smegmatis, wherein the organic acids were amixture of salicylic acid and lactic acid (at 0.4% weight and 2.2%weight, respectively, based on total weight of the formulation), and theamino acid based surfactant was sodium sarcosinate.

TABLE 4 Formulation E F G Ingredients On 100% On 100% On 100% C1-8Organic acids 2.0 2.0 2.0 Hydrogen peroxide 0.5 0.5 0.5 Sodiumsarcosinate 0.45 0 0.45 PnB 0 3.8 3.8 Sodium xylene sulfonate 0.3 0.30.3 Water Bal. Bal. Bal. Micro Efficacy against 0.57 4.51 6.07 M.smegmatis (Log Reduction)

Example 5

Table 5 further demonstrated the synergistic effect between organicacids and amino acid based surfactant against M. smegmatis under the EPAstandard according to the OECD Quantitative Methods for Evaluating theActivity of Microbicides. The organic acids were a mixture of salicylicacid, lactic acid, and formic acid (at 0.3% weight, 1.9% weight, and1.0% weight, respectively, based on total weight of the formulation).The amino acid based surfactant was sodium sarcosinate, and thestabilizing agent was PnB.

Formulation K showed that the high efficacy against M. smegmatis wereachieved even without the use of hydrogen peroxide in the formulation.

TABLE 5 Formulation H I J K Ingredients On 100% On 100% On 100% On 100%C1-8 Organic acids 0 3.2 3.2 3.2 Hydrogen peroxide 1.0 1.0 1.0 0 Sodiumsarcosinate 1.5 0 1.5 1.5 PnB 3.8 3.8 3.8 3.8 Sodium xylene sulfonate0.3 0.3 0.3 0.3 Water Bal. Bal. Bal. Bal. Micro Efficacy against 0.384.13 6.13 6.13 M. smegmatis (Log Reduction)

Example 6

Table 6 demonstrated a synergistic effect between C1-8 organic acids andamino acid based surfactant against Candida albicans under the standardtest EN13624, wherein the organic acids were a mixture of lactic acidand formic acid, the amino acid based surfactant was sodium sarcosinate,and the stabilizing agent was ethanol.

TABLE 6 Formulation L M N O P Ingredients On 100% On 100% On 100% On100% On 100% Organic Acids 5.1 0 0.5 5.4 8.1 on 100% active Sodiumsarcosinate, 0 9 9 9 9 30% Ethanol, 95% 5.2 5.2 5.2 5.2 5.2 Glycerine,87% 1 1 1 1 1 SLES 2EO, 28%⁽¹⁾ 9 9 9 9 9 SLS, 30% 9 9 9 9 9 Water Bal.Bal. Bal. Bal. Bal. Micro Efficiency <1 <1 1.38 2.00 4.18 againstCandida Albicans (Log reduction) ⁽¹⁾SLES 2EO is Sodium lauryl ethersulfate, 2EO ⁽²⁾SLS is Sodium laureth sulfate

Example 7

Table 7 showed an improved stability of the disinfectant formulationsupon including ethanol as a stabilizing agent in the formulations,wherein the disinfectant formulations comprised a mixture of lactic acidand formic acid as the C1-8 organic acids, and sodium sarcosinate as theamino acid based surfactant. Formulation Q, which did not include anyethanol stabilizing agent, was an unstable cloudy solution that resultedin a phase separation. Upon including ethanol stabilizing agent in theformulations (Formulations R and S), the stable clear solutions wereachieved.

TABLE 7 Formulation Q R S Ingredients On 100% On 100% On 100% SLES2EO/3EO 1.0 1.0 1.5 SLS 1.0 1.0 1.5 Sodium sarcosinate 3.5 3.5 4.0Glycerine 0.9 0.9 0.9 Lactic acid 8.0 8.0 7.0 Formic acid 1.0 1.0 0.0Ethanol 0 0.5 0.5 Water Bal. Bal. Bal. Appearance Cloudy solution Clearsolution Clear solution Stability Phase separation Stable Stable

Example 8

Disinfectant Formulation T comprised a mixture of lactic acid and formicacid as the C1-8 organic acids, sodium sarcosinate as the amino acidbased surfactant, and ethanol as the stabilizing agent.

Formulation T Amount Ingredient % Active Weight (g) % Weight Water 100% 61.0 61.00 SLES 2EO/3EO 28% 9.0 2.52 SLS 30% 9.0 2.70 Sodium Sarcosinate30% 9.0 2.70 Glycerine 87% 1.0 0.87 Lactic acid 88% 4.7 4.14 Formic acid85% 1.10 0.94 Ethanol 95% 5.2 4.94 100.0 100.00%

Table 8 showed the physical stability and antimicrobial stability datafor Formulation T at 40° C. storage. Formulation T was physical stableand maintained its antimicrobial activity for at least three monthsduring storage.

TABLE 8 Stability period Micro Efficacy Against (at 40° C. pH Candidaalbicans storage) Appearance (range 2.3 to 2.8) (EN13624, log reduction)0 day Clear, stable liquid 2.70 > 4 1 month Clear, stable liquid 2.70 >4 3 month Clear, stable liquid 2.78 > 4

Example 9

Table 9 showed the micro efficacy of the tested disinfectantformulations against Candida albicans under the standard test EN13624.

TABLE 9 Formulation V W X Y Z Ingredients On 100% On 100% On 100% On100% On 100% Alkyl ether sulfate salts 0.95-1.05 2.5-3.0 2.5-3.00.95-1.05 2.0-2.5 Alkyl sulfate salt 0.9-1.0 2.5-3.0 2.5-3.0 1.5-2.0~2.5 Alpha olefin sulphonates 1.1-1.2 0.9-1.0 0.9-1.0 0.9-1.0 0 Aminoacid based 0 0 1.5-2.5 1.5-2.5 2.4 surfactant C1-8 Organic acids9.5-10.5 9.5-10.5 0 10-11 ~4 (including alpha hydroxy acid) Alcohols,glycols, ~5 ~5 ~5 ~5 ~5 glycerols, glycol ethers Hydrogen peroxide 0 0 00 0 0.5-1.5   Demineralized Water Bal. Bal. Bal. Bal. 0 Micro Efficacyagainst <2 <2 <2 >4 >4 Candida albicans (Log Reduction)

Formulation V, which comprised C1-8 organic acids as the antimicrobialactive but without amino acid based surfactant, provided a log reductionof less than 2 against Candida albicans. No significant improvement inthe antimicrobial activity was observed even when the amount of anionicsurfactant was increased (Formulation W). Formulation X, which comprisedamino acid based surfactant but without C1-8 organic acids, provided alog reduction of less than 2. Formulation Y, which comprised both C1-18organic acid and amino acid based surfactant, provided the desiredantimicrobial efficacy (>log 4 reduction against Candida albicanspassing EN13624). These results demonstrated the synergy between theamino acid based surfactant and the C1-8 organic acid in achieving aneffective level of antimicrobial activity against Candida albicans.

Furthermore, Formulation Z demonstrated that upon including hydrogenperoxide (along with C1-18 organic acids, amino acid based surfactant,and stabilizing agent), the amount of C1-18 organic acids required toachieve the desired antimicrobial efficacy could be significantlyreduced.

Example 10

Table 10 below showed that the disinfectant compositions of FormulationDD was obtained at high level of salicylic acid (e.g., above 2% weightbased on total weight of the formulation), with high stability andexcellent antimicrobial efficacy. The micro efficacy of the disinfectantformulation was determined based on the EPA standard according to theOECD Quantitative Methods for Evaluating the Activity of Microbicides.

TABLE 10 Formulation AA BB CC DD Ingredients On 100% On 100% On 100% On100% Salicylic acid 0.8 0.8 1.6 2.4 Hydrogen peroxide 4.25 4.25 4.254.25 Phosphoric acid 3.4 3.4 3.4 3.4 Sodium sarcosinate 0 6.7 6.7 6.7Alcohol (C6-C12) 5 5 0 0 ethoxylate (3.5 EO) Alkyl diphenyloxide 0 0 3.83.8 disulphonic acid Na salt Ethanol 0 0 20 20 Water Bal. Bal. Bal. Bal.Physical Stability Clear phase Clear Clear separation Dilution 1:16 1:161:16 1:16 Fungicidal Efficacy 4.32 N/A 5.60 5.60 against T.interdigitale (Log reduction)

Example 11

Table 11 demonstrated the synergistic effect between stabilizing agentand amino acid based surfactant against S. aureus. The amino acid basedsurfactant was sodium sarcosinate, and the stabilizing agent was PnB.The micro efficacy of the disinfectant formulation was determined basedon the EPA standard according to the OECD Quantitative Methods forEvaluating the Activity of Microbicides.

TABLE 11 Formulation EE FF GG Ingredients On 100% On 100% On 100%Organic acids 0 0 0 Hydrogen peroxide 0.5 0.5 0.5 Sodium sarcosinate 1.50 1.5 PnB 0 3.8 3.8 Sodium xylene 0.3 0.3 0.3 sulfonate Water Bal. Bal.Bal. Micro Efficacy against 0.23 0.16 0.68 S. aureus (Log Reduction)

Various features and advantages of the invention are set forth in thefollowing claims.

We claim:
 1. A synergistic disinfectant composition, comprising: aminoacid based surfactant chosen from sodium sarcosinate of C10-24 fattyacid, sodium glycinate salt of C10-24 fatty acid, sodium glutamate ofC10-24 fatty acid, or any combination thereof, in an amount of fromabout 0.1% to about 20.0% by weight; C1-8 organic acid in an amount offrom about 0.1% to about 25.0% by weight; and hydrogen peroxide in anamount of at least 0.1% but less than 5.0% by weight, all based on totalweight of the composition, wherein the composition shows a synergisticantimicrobial effect between the amino acid based surfactant and theC1-8 organic acid.
 2. The disinfectant composition of claim 1, whereinthe composition further comprises stabilizing agent chosen from C1-6alcohol, glycerol, polyol, glycol ether, or any combination thereof. 3.The disinfectant composition of claim 2, wherein the composition hassubstantially the same antimicrobial activity after at least one monthat 40° C. storage.
 4. The disinfectant composition of claim 2, whereinthe stabilizing agent fulfills at least one of the following: (a) thestabilizing agent is present in an amount of from about 0.05% to about20.0% by weight based on total weight of the composition, (b) thestabilizing agent is present in an amount of from about 1.0% to about6.0% by weight based on total weight of the composition.
 5. Thedisinfectant composition of claim 1, wherein the composition furthercomprises anionic surfactant.
 6. The disinfectant composition of claim5, wherein the anionic surfactant comprises an alkyl sulfate, an alkylether sulfate, an alkyl aryl sulfonate, an alpha-olefin sulfonate, analkali metal or ammonium salt of alkyl sulfate, an alkali metal orammonium salt of alkyl ether sulfate, an alkyl phosphate, a siliconephosphate, an alkyl glyceryl sulfonate, an alkyl sulfosuccinate, analkyl taurate, an acyl taurate, a sulfoacetate, an alkyl phosphateester, a mono alkyl succinate, a monoalkyl maleate, a sulfoacetate, anacyl isethionate, an alkyl carboxylate, a phosphate ester, asulfosuccinates, or any combination thereof.
 7. The disinfectantcomposition of claim 5, wherein the anionic surfactant comprises analkyl sulfate, an alkyl ether sulfate, an alkyl aryl sulfonate, analpha-olefin sulfonate, or a combination thereof.
 8. The disinfectantcomposition of claim 5, wherein the anionic surfactant is present in anamount of from about 0.1% to about 20.0% by weight based on total weightof the composition.
 9. The disinfectant composition of claim 1, whereinthe amino acid based surfactant comprises sodium lauroyl sarcosinate,sodium cocoyl glutamate, sodium lauroyl glutamate, sodium lauroylglycinate, sodium cocoyl glycinate, or a combination thereof.
 10. Thedisinfectant composition of claim 1, wherein the amino acid basedsurfactant is present in an amount of 0.1% to about 3.0% by weight basedon total weight of the composition.
 11. The disinfectant composition ofclaim 1, wherein the C1-8 organic acid comprises formic acid, aceticacid, propionic acid, butyric acid, isobutyric acid, valeric acid,isovaleric acid, caproic acid, heptanoic acid, benzoic acid, salicylicacid, caprylic acid, lactic acid, glycolic acid, citric acid, malicacid, mandelic acid, tartaric acid, or any combination thereof.
 12. Thedisinfectant composition of claim 1, wherein the composition fulfills atleast one of the following: (i) the disinfectant composition comprisestwo or more types of the C1-8 organic acids, (ii) the disinfectantcomposition comprises from 0.3% to 15.0% by weight of the C1-8 organicacid based on total weight of the composition.
 13. The disinfectantcomposition of claim 1, wherein the composition has a pH of 5 or less.14. The disinfectant composition of claim 1, wherein the compositioncomprises, all based on total weight of the composition: the amino acidbased surfactant chosen from sodium sarcosinate of C10-24 fatty acid,sodium glycinate salt of C10-24 fatty acid, sodium glutamate of C10-24fatty acid, or a combination thereof, in an amount of from about 0.1% toabout 3.0% by weight; the C1-8 organic acid in an amount of from about0.1% to about 15.0% by weight; hydrogen peroxide as the oxidizing agentin an amount of at least 0.1% but less than 5.0% by weight; the anionicsurfactant in an amount of from about 0.1% to about 10.0% by weight;stabilizing agent chosen from C1-6 alcohol, glycerol, polyol, glycolether or any combination thereof, in an amount of from about 1% to about6% by weight; and optionally, at least one of a pH adjusting agent, abuffering agent, a nonionic surfactant, a hydrotrope, a corrosioninhibitor, a sequestering agent, an antimicrobial compound, a dye, arheology modifier, a preservative, or any combination thereof.
 15. Aconcentrate disinfectant composition that, when diluted with water,produces the synergistic disinfectant composition of claim
 1. 16. Adiluted disinfectant composition obtained by diluting the synergisticdisinfectant composition of claim 1 with water.
 17. A disinfectant wipecomprising the synergistic disinfectant composition of claim 1 embeddedin a textile medium.
 18. The disinfectant composition of claim 1,wherein the composition is in a form of liquid.